Portrait™ 630 Reagent Multi-Spotter
Introducing the Portrait 630™ reagent multi-spotter
The latest innovation from Labcyte Inc., the experts in acoustic droplet ejection! The Portrait 630 reagent multi-spotter is a fully automated non-contact, nozzle-free acoustic droplet ejection system optimized to deposit MALDI matrix and other reagents onto tissue sections and other samples for precision MALDI imaging mass spectrometry. Precise and flexible spot-on-spot positioning and timing enable sequential reaction chemistry and improve crystal formation, yielding higher quality, more sensitive, and more reproducible mass spectra.
Flexible and Fast
The Portrait 630 reagent multi-spotter gives you control over your experiments. The target holder accommodates both glass and metal targets in a variety of sizes from most of the major mass spectrometer vendors. The full-color CCD imaging system captures images of stained or unstained tissue so that you can indicate exactly where you want to apply reagents and matrix. You specify the number of droplets per spot, the number of repeat cycles, and the timing between droplets and cycles. You can create different biochemical conditions on different areas of the sample, or perform biochemical reactions by applying reagents sequentially to the same locations. Optimize experimental conditions in a single step (Figure 1). The Portrait 630 is fast—one cycle of one droplet per spot takes less than two minutes!
The latest innovation from Labcyte Inc., the experts in acoustic droplet ejection! The Portrait 630 reagent multi-spotter is a fully automated non-contact, nozzle-free acoustic droplet ejection system optimized to deposit MALDI matrix and other reagents onto tissue sections and other samples for precision MALDI imaging mass spectrometry. Precise and flexible spot-on-spot positioning and timing enable sequential reaction chemistry and improve crystal formation, yielding higher quality, more sensitive, and more reproducible mass spectra.
Flexible and Fast
The Portrait 630 reagent multi-spotter gives you control over your experiments. The target holder accommodates both glass and metal targets in a variety of sizes from most of the major mass spectrometer vendors. The full-color CCD imaging system captures images of stained or unstained tissue so that you can indicate exactly where you want to apply reagents and matrix. You specify the number of droplets per spot, the number of repeat cycles, and the timing between droplets and cycles. You can create different biochemical conditions on different areas of the sample, or perform biochemical reactions by applying reagents sequentially to the same locations. Optimize experimental conditions in a single step (Figure 1). The Portrait 630 is fast—one cycle of one droplet per spot takes less than two minutes!
Figure 1. Example of one-step protocol optimization on a single tissue section: MALDI matrix was spotted onto a rat brain coronal section in four overlapping tiled grids (1-4). Each section contains increasing amounts of matrix.
Spot-on-Spot Precision
Precise spot-on-spot placement prevents biomolecules from migrating throughout a large wetted area and ensures accurate positional information from the tissue (Figure 2). Apply the same or different reagents to the same spots multiple times. By controlling the drying time between droplets, you can optimize solvent penetration, reaction chemistry, crystal formation, and spot size to yield higher quality and more-reproducible mass spectra and images.
Precise spot-on-spot placement prevents biomolecules from migrating throughout a large wetted area and ensures accurate positional information from the tissue (Figure 2). Apply the same or different reagents to the same spots multiple times. By controlling the drying time between droplets, you can optimize solvent penetration, reaction chemistry, crystal formation, and spot size to yield higher quality and more-reproducible mass spectra and images.
Figure 2. Positional precision of the Portrait 630 reagent multi-spotter. Scanned image of spots at 500 µm spacing on clean metal target. Average spot diameter = 141 µm, CV=4.7%. Standard deviation of X position = 8.8 µm, standard deviation of Y position = 8.7 µm. (Specification = ±40 µm.)
Brochure Download
For further information on the Labcyte Portrait™ 630 Reagent Maldi-Spotter please download the following Brochure.
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info request form or send us an email.
Alternatively you can visit Labcyte at http://www.labcyte.com
info request form or send us an email.
Alternatively you can visit Labcyte at http://www.labcyte.com