XF96 Extracellular Flux Analyzer


The XF96 Extracellular Flux Analyzer is a fully-integrated instrument that simultaneously measures the two major energy yielding pathways - aerobic respiration and glycolysis - in a convenient, microplate format.

This fast and sensitive measurement of cellular bioenergetics is label free, enabling time-resolved analysis and the reuse of the cells. XF assays provide increased throughput in a drug discovery format that is superior to its single parameter predecessors.

By incorporating low cost, disposable fluorescent-based optical sensors, multiplexed fiber optics and highly integrated electronic components, XF instruments offer scientists the ability to:
  • Simultaneously measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in as little as five minutes
  • Perform time-resolved measurements of a single population of cells over a period of minutes, hours or even days
  • Re-use cell samples after completing an XF measurement

A Novel, Integrated System

The XF96 Extracellular Flux Analyzer is a highly-sophisticated, yet easy-to-use instrument comprised of:

Bench-top Analyzer
The XF Analyzer fits easily on a bench top and requires only a standard lab power connection. An internal measuring chamber is maintained at 37 degrees Celsius at all times, and plates can be loaded manually, or a robotic plate handler and fluidics system can be used to develop automated assay protocols.

User-friendly Controller Interface
A high-resolution, color LCD touch screen provides a rich user interface for increased productivity and shorter assay running times. Integrated with the XF Analyzer, the networkable controller comes loaded with a Microsoft operating system for easy use.

Portable PowerGrid Acquisition & Analysis Software
XF software supports the way that you work. Experiments can be designed on any desktop computer in a spreadsheet format within Microsoft Excel®. In the lab, the XF Analyzer populates assay results into this spreadsheet. The user can then analyze data either on the XF instrument touch screen, or on a desktop computer.

XF data can be viewed in raw form, analyzed using Excel, or analyzed using supplied Seahorse data review and charting tools. The standard XF PowerGrid desktop software tools are provided with an unlimited-use license for your organization.

Convenient FluxPaks
Each XF96 Analyzer comes with two FluxPaks which contains the correct ratio of XF Assay Kits, XF Cell Culture Plates and XF Calibrant necessary to perform up to eighteen 96-well assays.

To request further Information on this product please use either the info request form or send us an email.

Alternatively you can visit Seahorse Bioscience, Inc. at http://www.seahorsebio.com