Seahorse XFp Extracellular Flux Analyzer


Determine the Metabolic Phenotype and Potential of Your Cells Within 1 Hour!

The Seagorse XFp Cell Energy Phenotype Test determines the metabolic phenotype of a cell to reveal its metabolic potential, a parameter that can only be identified using Seaorse XF Technology. The metabolic phenotype and metabolic potential combine to exhibit the energy phenotype of the cell, which then enables functional comparisons between groups.  


Baseline Phenotype

Measurement of the cells relative utilization of mitochondrial respiration and glycolysis under starting conditions.

Stressed Phenotype
Measurement of the cells' relative utilization of aerobic respiration and glycolysis when stressed.

Metabolic Potential
Measurement of the cells' ability to meet a stress demand via mitochondrial respiration and glycolysis.

(click on image for a larger view)

Seahorse XFp Cell Energy Phenotype Test Kit

Seahorse XFp Cell Energy Phenotype Test Kit

The only method to measure the metabolic phenotype and potential of your cells


Cells have distinct metabolic phenotypes that reflect the relative utilization of both major energy pathways under different conditions. An understanding of this balance provides researchers insight into the functional consequences of biological differences between groups.

By simultaneously assessing mitochondrial respiration and glycolysis under both basal and stressed conditions, the Seahorse XFp Cell Energy Phenotype Test can determine the metabolic phenotype of cells and reveal their metabolic potential. These parameters combine to reveal the energy phenotype of a sample.
  • Rapidly measures the relative utilization of both major energy pathways in live cells in real time.
  • The only method that can provide a direct measurement of ‘metabolic potential’.
  • Easy-to-use kit that provides the Gold Standard measure of metabolic function.
(for use with the Agilent Seahorse XFp Analyzer only)


By combining quality controlled reagents with real time, label-free detection provided by the Seahorse XFp Analyzer, the Seahorse XFp Cell Energy Phenotype Test Kit quickly measures mitochondrial respiration and glycolysis under both basal and stressed conditions. The dedicated Report Generator automatically determines the 'energy phenotype' and 'metabolic potential' of your samples, simplifying data analysis and interpretation.


Proven XF Technology, Accessible for Every Lab

The Seahorse XFp Extracellular Flux Analyzer is built on proven Seahorse XF technology for monitoring the two major energy pathways of the cell:  Oxidative Phosphorylation, measured by Oxygen Consumption Rate (OCR) and Glycolysis, measured by Extracellular Acidification Rate (ECAR).

The Agilent Seahorse XFp Analyzer performs the gold standard Seahorse XF assays on small sample sets with ease, making it ideal for:
  • Pairwise comparisons
  • Patient-derived samples
  • Personal use

Benefits of the Agilent Seahorse XFp Analyzer

  • Allows quick assessment of a few samples
  • Runs standard assays without need for special bioenergetics expertise
  • Affordable by individual labs

XFp Miniplate and sensor cartridge
For researchers analyzing precious, limited biomaterial or who just prefer to run a few samples per day, the XFp Analyzer provides powerful XF technology in a streamlined, easy-to-use platform.

The Seahorse XFp Analyzer, Seahorse XFp FluxPaks and Seahorse XFp Miniplates provide a compact, affordable format for performing standard Seahorse XF assays such as the Seahorse XF Cell Mito Stress Test, Seahorse XF Glycolysis Stress Test, and Seahorse XF Metabolic Switching. With six assay wells, the Seahorse XFp Miniplate has the same well geometry as the Agilent Seahorse XFe96 microplate.

Key Applications for the XFp Analyzer

Seahorse XF technology delivers functional metabolic parameters that have become the gold standard for energy metabolism.  With the XFp Analyzer it is easier than ever to complete your research story with these standard assays:

XF Glycolysis Stress Test
Analyze Metabolic Reprogramming in Primary Immune Cells

In order to respond to foreign antigens, immune cells must be able to increase their metabolism.  In these mouse bone-marrow derived macrophages, overnight activation by LPS revved up the glycolytic machinery.  The XFp detected an increase in both glycolysis (ECAR) and glycolytic reserve compared to quiescent cells.

XF Cell Mito Stress Test
Verify impact of gene expression changes on mitochondrial function

Oxidative phosphorylation (OXPHOS) requires the mitochondrial electron transport chain as well as access to substrates such as pyruvate.  Inhibition of mitochondrial pyruvate carrier 1 (MPC1) by UK5099 has no impact on basal OXPHOS but spare respiratory capacity is abrogated in C2C12-derived myotubes.

XF Metabolic Switching
Detect the Warburg Effect in a single pairwise experiment

Upon induction of certain signaling pathways, cells will shift their mode of energy production.  Activation of HIF-1α by CoCl2 induces a metabolic switch in SKOV-3 cancer cells.  The XF Phenogram shows that OXPHOS (OCR) of the treated cells is reduced and glycolysis (ECAR) is increased relative to the control.

Cellular Bioenergetics Webinars

Seahorse Bioscience Cellular Bioenergetics Webinar Series featuring key thought leaders discussing their areas of expertise and the Seahorse XF technology.

This page lists all of Seahorse's upcoming and past webinars. You may also view Seahorse's on-Demand webinars.

Seahorse Video Library

Videos explaining how the Agilent Seahorse XF Analyzer technology works are available at www.agilent.com

To request further Information on this product please use either the info request form or send us an email.

Alternatively you can visit Seahorse Bioscience, Inc. at www.seahorsebio.com