NovoCyte Flow Cytometer

High Performance Flow Cytometer for Everyone

NovoCyte® is a high performance benchtop flow cytometer designed for all levels of users and all types of laboratories. This budget-friendly instrument is capable of detecting up to 15 parameters with enhanced sensitivity and resolution. The customizable laser and optical configurations of NovoCyte offer a high degree of flexibility while providing complex cell analysis capabilities. The NovoExpress® software facilitates easy and intuitive sample acquisition and analysis. Automation of multiple fluidic functions eliminates cumbersome and time consuming procedures. User hands-on time is minimized by the flexible NovoSampler® Pro which can automatically analyze samples in single tubes, multi-tube racks, or 24-, 48-, or 96-well plates.


Address the full range of current and future multi-parameter analysis research needs with the NovoCyte flow cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform.

ACEA offers a system which is:
  • Powerful- up to 15 parameter detection with enhanced sensitivity and resolution.
  • Intuitive – automated instrument maintenance functions and advanced data analysis capabilities for greater usability.
  • Customizable – 1 to 3 laser options, exchangeable filters, multiple sampling options, and flexible analysis formats.
Other important features enhance usability, such as fixed optical alignment, 24-bit detection dynamic ranges with no need for PMT voltage adjustment, accurate volumetric-based cell counting, pressure sensors to monitor fluidic status in real-time, and automatic cleaning and decontamination processes.

ACEA also developed the NovoExpress software, which is intuitive and easy to use. Flexible analysis templates and plotting tools offer enhanced data analysis efficiency.

Configurable Laser Systems

ACEA NovoCyte Video

Novocyte Features

Autosampler Option

Option for High-Throughput and Automated Analysis of Multiple Samples

The NovoSampler is a modular autosampler, that can be interchanged with the single tube loader in just a couple minutes, is compatible with 12 x 75mm tube, 1.5 & 2mL tubes and 24/48/96 well plates, allowing for versatile loading modes, and increased throughput. Automatic calibration is just the push of a button and this procedure ensures accuracy between different plate types. Simply select your plate, identify your wells or tubes to run, and start running. The NovoSampler will automatically switch between samples, employ mixing or rinsing steps between samples if desired, and complete your run all while you are away.


Optimized PMT Voltage & 7 Decades Detection Dynamic Range
Provides 107 dynamic range for signal detection and processing, offering a broader signal range than other flow cytometers. Wide range of fluorescent and scattering signal intensities eliminates the need for complicated and laborious PMT voltage adjustment. Data acquisition is just load-and-go.

Configurable Fluorescence Detection Channels for Enhanced Assay Flexibility
Customizable selection and upgrade with lasers of different wavelengths allow for personalized, versatile choices of fluorochromes for flow cytometry assays. User interchangeable filters and dichroic mirrors broaden available fluorescence channels to expand users’ detection options.

13 Fluorescent Channel Detection: Multiple Laser Options Providing Flexibility of Choice (405nm, 488nm, 561nm, 640nm)
Customize the instrument to meet detection needs with blue, red and violet laser options. System can be upgraded as analytical requirements increase – no need to purchase another instrument. State-of-the-art solid-state lasers provide high quality and extremely stable optical illumination.

Novel Optical Design & Enhanced Signal Detection
Patent pending independent beam shaping optics and light collection system maximizes signal detection efficiency, increasing sensitivity and resolution for each fluorescence channel. Fixed optical alignment removes need for daily maintenance and adjustment of optical system, providing convenient operation and superior long-term stability. Optimized forward scatter obscuration bar increases sensitivity. Enhanced small particle detection and resolution as a result of optimized obscuration bar design.


Volumetric Fluidics System for Increased Accuracy & Easy Maintenance
The NovoCyte flow cytometer uses a highly accurate, volumetric syringe pump which enables direct absolute counting of samples, eliminating the need to use expensive reference beads.

Real-time monitoring of fluidics prevents flow path obstruction. Accurate pressure sensors monitor fluidic status in real-time, thus the possibility of flow path obstruction is greatly reduced, allowing for reliable and accurate data acquisition. Automated SIP (sample injection probe) washing following sample acquisition minimizes sample carryover and increases capability to detect rare events. High speed data acquisition of 35,000 events per second.

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