SmartPath™ Technology

DeNovix UV-Vis spectrophotometers accurately measure 0.5 – 1.0 uL samples utilizing patent-pending SmartPath® Technology. The opto-mechanical components and algorithms provide ultra-precise, real-time pathlength control to deliver performance unmatched by any microvolume spectrophotometer. The DS-11 continually compresses a sample during measurements rather than stretching. This method is especially beneficial for quantification of low surface tension fluids such as protein samples.

Always Calibrated
The DS-11 does not rely on fixed path length calibrations and therefore does not require regular calibration checks. There is no downtime or expense required for routine calibration like many other spectrophotometers. DeNovix offers a LC-NA verification standard for labs that may need to verify proper operation from time to time in accordance with their own quality standards.

Bridge Testing™

DeNovix software includes a proprietary algorithm to test for an unbridged condition in the primary nucleic acid and protein quantification apps. This element of the SmartPath® algorithm not only detects the broken liquid column but compensates to ensure accurate results with no user action required. Learn more in TechNote 135.

Truly 1.0 uL Sample Volumes
A key feature of the DS-11 is that the longest path length used (0.5 mm path) is shorter than the 1.0 mm path used by other microvolume spectrophotometers. This enables the DS-11 to truly measure 1.0 uL samples, virtually eliminating the possibility of erroneous results due to sample column breakage.

Automatic Pathlength Adjustment
Many microvolume spectrophotometers may offer multiple pathlengths, but many require the user to guess which pathlength is needed prior to measuring the sample. The DS-11 uses real-time absorbance data to determine the best path length (ranging from 0.03-0.5 mm) to use for each measurement.

Ultra High Absorbance
The DS-11 can measure samples with absorbance values as high as 500 absorbance units (at a 1 cm equivalent path length). This translates into 750 mg/ml of BSA protein or 25000 ng/uL of dsDNA. This ultra high absorbance capability is achieved by using a remarkably small path length of 0.03mm (30 microns) enabled by our SmartPath® Technology. Even at this short path length, typical CV of measurements is less than 3%.

Superb Precision

The reproducibility of measurements on the DS-11 is an important performance feature. For example, replicates of 500 ng/ul dsDNA samples typically result in CV’s of less than 1%.