HD Imaging

HD Imaging is a proprietary imaging format developed by Essen specifically for IncuCyte™. HD is a phase imaging technique which is largely insensitive to fluid-meniscus artifacts. HD imagery looks a little different than standard phase contrast imagery as shown in the accompanying images. In general, HD shows more cell detail and less of a halo, but the biggest difference is that the cells that have rounded up appear dark, as opposed to bright.

HD Imaging was developed in response to the poor image quality inherent with traditional phase contrast optics in 96 and 384-well microplates. The poor quality is due to aberrations produced by the fluid-meniscus. HD imaging is largely insensitive to fluid-meniscus artifacts.

HD enables high-contrast, non-labeled imaging in microplates providing morphological information difficult to attain with fluorescent-only imaging. Essen is developing microplate-based kinetic imaging assays based on this technology.