Fluxion Biosciences Inc.

Company Overview

Fluxion Biosciences was formed in 2005 to address critical needs for cellular analysis tools in the pharmaceutical, biotech and research industries. The company has developed a proprietary microfluidic platform which enables precise cellular manipulation, rapid low-volume compound addition, environmental control and software-based data acquisition.

Fluxion's lead applications in development include the following Systems:

Technology Overview

Well Plate Microfluidics™ technology provides numerous benefits for cellular assays

Fluxion’s technology platform is based on application-specific microfluidic designs and benchtop control instruments.  Each implementation addresses a specific cell-based assay.  The patented microfluidic device designs are seamlessly integrated with standard well-plate formats, providing ease of use and compatibility with existing fluid handling equipment.

A key feature is the ability to interface to any number of channels simultaneously, which allows accurate control of flow velocities and electric field application throughout the microfluidic network. This enables many experiments to be run simultaneously.  At the conclusion of one experiment, a new preloaded device can be connected to the multichannel interface in a matter of seconds.

The devices are produced by micromolding, with molds fabricated using silicon photolithography techniques.  Photolithography, a technique adapted from the semiconductor industry, uses the application of high energy photons through predetermined patterns (mask set) to thin layers of photoresist layers on the mold surface.  Exposure leads to selective cross-linking of the photoresist, and a chemical development step washes away unwanted material.  The resulting features have sub-micron resolution and are used as molds to produce the polymeric well plate.  Microfluidic designs can be tailored to a wide range of functional cellular assays.

The high level of flexibility and low experimental cost are enabled by the fact that all active elements (valves, electrodes, imaging, etc.) are kept off-chip, with fluidics supplying the critical functionality of the system: accurate flow control, cell manipulation and localized compound application.