Protein Quantitation in Bioprocessing

Label-free technologies offer an ideal solution for reducing the bottlenecks in bioprocessing to ensure high product quality and improve efficiencies. ForteBio’s Octet family of instruments provides rapid and accurate analysis of antibody concentrations and other proteins. Its analytical capabilities provide particular value in applications where existing methods such as HPLC and ELISA have limitations in throughput, performance, workflow, and ease of use.

Figure 1: Octet QK correlation with HPLC

With full-plate antibody quantitation in 32 minutes, BLI-based instruments provide label-free quantitation of antibodies or other proteins that are critical to:

• Early cell culture screening
• Protein purification
• Cell line selection for optimization of antibody production
• Biologicals manufacturing monitoring

Accurate antibody quantitation is critical to the selection of cell lines for development and the optimization of bioreactor titers in production. Traditional measurement methods include ELISA, HPLC, nephelometry, and densitometry. Drawbacks to these methods include long analysis times, lack of specificity, labor-intensive protocols, and variable imprecision. The BLI-based Octet family of instruments supports each step of bioprocessing, streamlining and speeding your workflow.

The Octet family of instruments provides a complete on-demand, label-free solution for antibody quantitation. Pre-coated anti-hIgG, anti-murine, and Protein A Biosensors are ready to use in quantitating a variety of IgG isotypes. Streptavidin-coated and Amine Reactive Biosensors expand your quantitation options to include all proteins in developing custom protein assays that are easier to process than ELISAs, while still providing rapid and accurate results.

Figure 2: Bioreactor process time profile of cell culture supernatants