BioLevitator 3D Cell Culture System

The BioLevitator™ is a benchtop incubator and bioreactor hybrid for up to four 50 ml high density 3D cultures. It utilizes the GEM™ technology which is based on specific magnetic microcarriers to which adherent cells attach.

By a switchable magnetic mechanism and smooth movement of the flasks the GEMs are kept in a floating state with the freedom to move through the entire flask space during growth, i.e. 3D cell culture. With the BioLevitator™, a number of procedures and equipment are eliminated from the classical cell culture workflow significantly simplifying and shortening the entire procedure.

• HD cell culture proven for a variety of adherent cell types, e.g. established cell lines as well as primary cells and stem cells

• streamlined workflow and reduced hands-on time: easy media exchange, washing and harvesting of cells

• elimination of manual working steps and peripheral instruments like centrifuges and incubators

• absolutely reproducible growth conditions for consistent experiments

• fully integrated environmental control (e.g. CO2, temperature)

• online measurement of relevant culture parameters (e.g. cell density, pH)

• stand-alone solution operated by a large touchscreen display

• easily integrated in Hamilton’s robotic platforms

• bead diameter between 75 and 150 µm

• increased adherent surface to volume ratio compared with standard cell culture dishes

• optically clear and non-autofluorescent – no interference with fluorescent and non-fluorescent imaging assays

• compressible and porous alginate core enhancing cell polarity and increasing in vivo like cell metabolism

• thin gelatine coating supporting the attachment of a wide range of cell types

• magnetic beads included in the alginate core for maneuvering of the GEM™s during media change, washing and harvesting