Drug Actions in Xenograft Fine Needle Aspirates

Fine needle aspirates (FNAs) taken from mouse xenografts typically contain too few cells for traditional protein analysis. The NanoPro 1000 has been used to successfully monitor a variety of signaling proteins in xenograft FNAs, which creates the possibility of following drug actions over time in a single mouse. The figure below shows NanoPro 1000 data generated from a xenograft formed following subcutaneous injection of leukemia cells into a SCID mouse. The Gleevec-induced decrease in STAT5 phosphorylation was dramatic, while the level of HSP70 loading control remained essentially constant.