NanoPro Technology

NanoPro technology provides a protein/phosphoprotein assay with many of the attributes of Western blot analysis — an assay that combines a physical separation of proteins with the specificity of antibody-based detection. Because of a unique capillary-based design, NanoPro assays are highly quantitative, seamlessly automated, and extremely sensitive. As a result, for the first time, researchers can analyze previously undetectable low-abundance proteins while conserving rare and precious samples such as stem cells, primary cells, and fine needle aspirates.

How does it work? As shown in the figure below, after the 384-well sample plate is loaded into the NanoPro 1000, the NanoPro assay is performed in six steps:

Probed capillary isoelectric focusing assays on the NanoPro 1000.

Step 1: Load
The capillary is filled with a 400-nL mixture of sample, fluorescently labeled pI standards and ampholytes.

Step 2: Separate
Voltage is applied across the capillary to drive the IEF separation. Individual proteins and pI standards concentrate at their isoelectric points, and the position of each standard in the capillary is recorded.

Step 3: Immobilize
The capillary is exposed to UV light, activating the proprietary linking chemistry and locking the separated protein isoforms to the capillary wall.

Step 4: Immunoprobe
The capillary is rinsed and immunoprobed for specific proteins. Luminol and hydrogen peroxide are added to catalyze the generation of chemiluminescent light, which is captured by a CCD camera.

Step 5: Quantitate
The digital image is analyzed and quantitative results are presented
in the software.

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Alternatively you can visit ProteinSimple at http://www.proteinsimple.com