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Acea Biosciences Inc.

NovoCyte® Advanteon™ Benchtop Flow Cytometer

Advancing the Boundaries of Flow Cytometry

The Agilent NovoCyte Advanteon flow cytometer builds upon the highly successful Agilent NovoCyte and NovoCyte Quanteon to provide an advanced set of capabilities for the most demanding scientists, yet is remarkably simple to operate. The NovoCyte Advanteon can accommodate today’s high-end and increasingly sophisticated multicolor flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors.

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NovoCyte Advanteon Flow Cytometer Features

The NovoCyte Advanteon can accommodate today’s high-end and increasingly sophisticated multicolor flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors. The NovoCyte Advanteon is customizable to meet your specific needs, and is easily upgradeable to meet your future demands. When throughput is essential, add Agilent NovoSampler Q to your laboratory automation platforms. It can efficiently process both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. For the NovoCyte Advanteon, the intuitive and industry-honored Agilent NovoExpress software is now even more advanced, providing an exceptional user experience in data acquisition, analysis, and reporting.

  • Expanded customization and upgrade potential with up to 21-color options using up to 3 lasers
  • Sample recovery mode serves to collect unused sample at end of acquisition
  • Superior sensitivity and resolution
  • Intuitive and powerful software for data acquisition, analysis, and reporting
  • Smart-design functionalities and walk-away operation simplifies your workflow
  • Automation-ready for high-throughput needs
  • Wide, 7.2-log dynamic range eliminates the need for routine detector adjustments
  • High-speed collection of up to 100,000 events/second
  • Accurate absolute cell count in every experiment, which eliminates the need for reference beads

The Ultimate Photodetector

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Silicon photomultipliers (SiPM) are solid-state, photon-level sensitive semiconductor devices. The innovative optical design with the NovoCyte Advanteon incorporates 21 independent SiPM, which collect light on each and every channel, with sensitivities that far exceed the traditional PMT’s.

SiPM's strengths:

  • Superior photon detection sensitivity
  • High gain and high quantum efficiency
  • Instantaneous warm up and fast response
  • Robust and long lifespan
  • High durability

Consistent results, fast or slow

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The fluidic feedback control mechanisms found on the NovoCyte Advanteon consistently maintain exceptionally steady flow rates. The superior stability across a wide range of sample flow rates provide consistent results under variable operating conditions. As shown below, the CV’s at different flow rates are dramatically improved over other systems on the market.

Advantage of Performance and Flexibility

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With up to 21 fluorescence channels, the NovoCyte Advanteon offers tremendous flexibility in panel design, thereby allowing more data to be collected per run. Assessment of T cell state and function is just one critical example for researching cell-based immunotherapies using multi-color staining panels. Here, the NovoCyte Advanteon was used to show T cell differentiation, monitor T cell exhaustion, and measure cytokine proliferation from PBMC’s following activation, all in one experiment.

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ACEA Biosciences, Inc.

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