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Agilent Technologies, Inc.

NovoCyte® Quanteon™ Benchtop Flow Cytometer

Four Lasers, 25 Colors Plus Scatter Parameters

The NovoCyte Quanteon™ is a breakthrough in flow cytometry design in that it provides 25 fluorescent channel capability, with light collected against 25 different detectors. Since the detectors have a 7.2 log dynamic range, coupled with advanced auto compensation features, the need to tune each detector has been completely alleviated. You now have the flexibility to choose from 25 fluorescent channels utilizing four lasers, without having to sacrifice the performance in one channel, for another channel.

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NovoCyte Quanteon Flow Cytometer Features

Built upon the tremendous success of the NovoCyte® line of flow cytometers, ACEA Biosciences now introduces the most advanced benchtop flow cytometer on the market today, the NovoCyte Quanteon™.

  • The Quanteon™ is a 4 laser flow cytometer that can be configured with up to 25 independent photomultipliers, thereby meeting the most demanding panels.
  • The proprietary photomultiplier technology employed in the Quanteon™ provides superior sensitivity and stability, along with a 7.2 log dynamic range. Very dim signals and very bright signals can all be captured and gated in the same view, thereby eliminating the need for laborious trial and error PMT tuning procedures. When developing large color panels, the Quanteon™ will save you hours of time and frustration.
  • Superior fluidics provide pulse-free sample delivery, with high reproducibility and exceptionally low CV’s. Absolute counts, without the use of expensive beads, will save you time and money.
  • Fully automated flow cell wash and shut down features, which mitigates against flow cell clogging. System is always ready-to-go for the next user.
  • Walk-away automation with the NovoSampler Q™. Provides superior shaking, even at ultralow volumes, accommodates the most popular plate formats, bar coding, and built-in API’s for lab automation systems.
  • Leverages the highly successful NovoExpress® software, which is considered one of the most popular software packages on the market. Auto-compensation features, cell cycle analysis modules, cell proliferation modules, heat maps, batch analysis, and much more, are all built-in to the NovoExpress® Software package. The NovoExpress® Software is renowned for its productivity attributes and especially for its ease-of-use. Even a novice can be up and running in under an hour.

The fluorescent channels available on the NovoCyte Quanteon™ from ACEA include:

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Advanced Optical Detection and Flexible Configuration

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The proprietary optical detection system found inside the NovoCyte Quanteon™ employs a series of 25 Silicon photomultipliers (SiPM), which have superb detection sensitivity, at the photon level. The novel design provides highly discrete management of each fluorescent channel, superior optical detection, and cross-talk between channels is completely minimized. The NovoCyte Quanteon™ also has an automatic optical filter recognition feature, which allows users to efficiently change filter configurations, as needed by the user. The system has a fail-safe hardware feature, which conveniently alerts the user to any improperly arranged filter configurations. This feature mitigates against potential hardware errors during system set-up, which makes it ideal for a multi-user environment.

Exceptional Reliability With Consistent Results, at both Fast or Slow Flow Rates

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Equipped with lasers, optical filters, detectors, and fluidics of the highest quality, the Quanteon™ provides consistent signal detection, with low CV’s.  When combined with superior fluidic feedback control mechanisms, the Quanteon™ maintains steady flow rates with remarkable consistency.  As shown below, the Quanteon™ provides superior stability across a wide range of sample flow rates and operating conditions, which is a critical requirement for any high end flow cytometer.  The NovoCyte Quanteon™ gives you peace of mind, so that you can focus more on your experiments, with the assurance that your Quanteon™ will perform admirably.

Walk-away Automations Capabilities

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  • Quick startup and pre-scheduled shutdown.
  • Hassle-free fluidics allows for automatic clog detection and recovery.
  • “Smart” optical filters are automatically recognized by the instrument to ensure correct configuration with easy filter swapping.
  • The fluidic station will signal low fluid or high waste levels, and eliminates the need of manual inspection. Fluidics consumption is estimated before you run a plate, which ensures uninterrupted sample acquisition.
  • An integrated bar code scanner is employed, which captures essential plate and sample information.
  • The Quanteon™ supports a variety of plate formats (40 tube rack, 24/48/96/384 well plates), as well as a feature for custom plates.
  • The NovoSampler Q™ is a high performance orbital shaker, that is completely programmable and ensures proper cell suspension, even at the small volumes found in the 384 well plate format.

Benefits of NovoCyte Flow Cytometry Automation – time and cost saving with trusted results 

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  • Flow Cytometry automation enables 24/7 instrument operation, allowing for resource optimization and shorter time-to-results
  • Manual sample-handlings are time-consuming and error-prone, integrated automation workflow offers trusted results and leads to cost savings
  • Complete automation solutions offer transformative simplicity, sustainable reliability, and exceptional walk-away convenience to bring the best user experience
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Luz M. Londoño, Halozyme Therapeutics Inc. (review from SelectScience)

“Amazing instrument, it is the future of flow cytometry!”

“I have only wonderful things to say about the Novocyte®. It is incredibly easy to use, we get reproducible results, the software is the best one out there, the maintenance is super easy, and ACEA has excellent technical support! Can’t wait to upgrade to the 25-channel instrument!“

Matthias Schiemann, Technische Universität München

“Software is straightforward, and the software interface is easy to handle”

“This (NovoExpress®) software is straightforward, and the software interface is easy to handle. The implemented auto compensation and hierarchic tree structure is a highlight for effective organization of experimental data.”

Joan Christiansen, H. Lundbeck A/S. (NovoCyte review from SelectScience)

“My best friend in the lab!!!”

“I use the NovoCyte® to screen compounds for induction of micronuclei in the TK6 cell line as part of our gene tox strategy. With the NovoSampler® and 96-well format, the assay is extremely robust and the results reproducible to a very high degree. The NovoCyte® is user friendly and creating new applications to support our immune tox strategy has been delightfully easy, even for a novice within the field of immunology.”



Agilent Technologies, Inc.

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