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Agilent Technologies, Inc.

Seahorse XF Real-Time ATP Rate Assay

The ATP rate measure you've been looking for

Measuring your cells' total ATP level provides you with a piece of the story. However, without a view on the rate of production or pathway contribution, you may be missing important insight into cell proliferation, differentiation, activation and death.

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The Agilent Seahorse XF Real-Time ATP rate assay allows you to measure what matters. Now you can measure the rate of ATP production from the two key energy pathways (glycolysis and mitochondrial respiration) simultaneously in live cells using label-free technology. Unlike traditional end-point total ATP assays, this real-time, dual-pathway kinetic quantification delivers a dynamic picture of cellular bioenergetics with pathway contribution indicators, delivering earlier and deeper insights over other cell analysis techniques.

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Quantify ATP production rate from both glycolytic and mitochondrial pathways.

Key outputs of Agilent Seahorse XF Real-Time ATP rate assays.

A: Illustrates quantitative metabolic shift from mitochondrial respiration to glycolysis, resulting in different phenotypes under different conditions.

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Monitor kinetic responses in ATP production rate from drug or compound dosing.

B: Shows the changes of mitoATP production rates and glycoATP production rates over a time course in one cell population. Check out the White Paper to learn how these measurements are obtained.

XF ATP Rate Assay Kit

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Seahorse XF Real-Time ATP rate assay kit for the Agilent XFe24 and XFe96 Analyzers.

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XFp ATP Rate Assay Kit

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Seahorse XFp Real-Time ATP rate assay kit for the Agilent XFp Analyzer.

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Resources

Downloads

Download the Agilent Seahorse XF Assay Kits & Reagents Overview
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Download the new Media Selection Guide
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Download the Seahorse Plastics Brochure
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Download the Seahorse XF Instrument Selection Guide
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Agilent Technologies, Inc.

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Seahorse XF Real-Time ATP Rate Assay

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