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Agilent Technologies, Inc.

Agilent Seahorse XF Pro Analyzer

Agilent Seahorse XF Pro Analyzers measure the oxygen consumption (OCR) and extracellular acidification rate (ECAR/PER) of live cells in a 96-well format. The XF Pro Analyzer features better OCR precision at low rates, verified instrument performance and repeatability specifications, optimized temperature control, and is automation enabled.

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  • Better precision at low OCR allows for the confident interrogation of additional immune cell types and cell types that are bioenergetically compromised
  • Verified instrument performance delivers verified CV% for mid-high OCR/PER rate ranges, ensuring consistent XF data from plate to plate
  • Reports real-time metabolic rates in minutes, without sample extraction or labeling
  • Four-port injection system with automated mixing function allows detection of live-cell responses to substrates, inhibitors, and other compounds in real time
  • Achieve high-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
  • Precision-controlled heating tray maintains 16–42°C temperatures (8–20°C above ambient conditions) for compatibility with a variety of sample types
  • XF Pro Controller software allows easy assay protocol creation and user guided interface for performing XF assays. Wave Pro Data Quality View automatically flags erroneous data and allows for easier rejection of outlier data points.



Automatic measurement of energy metabolism in real time

Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.


Discover the Power of Cellular Metabolism

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The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. Discover how bioenergetic parameters can provide powerful insights and can serve as an indicator for disease models, critical cell processes, and therapeutic discoveries.

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Immune Metabolism

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Immune cell processes including activation, proliferation, and memory development are all driven by metabolic reprogramming, which can be tuned to enhance performance and control immune cell outcomes. Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements.

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Cancer Metabolism

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Metabolism is a critical driver of cancer malignancy, with cancer cell proliferation commonly requiring an upregulation or “metabolic switch” towards a more glycolytic phenotype to fuel the rapid energy requirements and generate building blocks for cancer cell growth. Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology.

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News & Events



BBT Webinar (recorded on 5. March 2024) on

Live Cell Metabolic Profiling, the Potential of Agilent Seahorse Analyzers and Assays

Join us for the second instalment of our webinar series, focusing on the dynamic world of live cell metabolic profiling using Agilent Seahorse Analyzers and Assays. Building upon our previous Luna Cell Counters webinar, we'll guide you through the potential of Agilent Seahorse Analyzers, providing insights into unraveling the intricacies of metabolic processes within live cells. Discover how these advanced tools not only provide valuable insights into cellular energetics but also offer a streamlined approach, saving you time and resources. Don't miss the opportunity to explore the forefront of live cell metabolic profiling with Agilent Seahorse, presented by experts in the field.



Download the Seahorse XF Instrument Selection Guide
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
Download the new Media Selection Guide
Download the Seahorse Plastics Brochure


Agilent Technologies, Inc.


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Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

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