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Agilent Technologies, Inc.

Agilent Seahorse XFe24 Analyzer

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

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Features

  • Real-time Results – this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37°C).
  • Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42°C (12°C-20°C above ambient conditions).
  • Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
  • Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
  • Easily create assay protocols and analyze data with Seahorse Wave software
  • For Research Use Only. Not for use in diagnostic procedures.

Technical specifications

Number of Assay Wells

20 - 22

Microchamber volume

7 µl

Controler

Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.

Software

Design and analyze assay templates on Controller or Wave desktop.

Best for

  • Islets
  • Larger samples
  • small model organisms

Key Advantages

  • Validated for hypoxia
  • Validated for assay temperatures 16-42°C (12–20°C lower ambient temp required)

Plate Format

24-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data

Multimedia

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News & Events

News

Resources

Downloads

Download the Seahorse XF Instrument Selection Guide
DOWNLOAD
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
DOWNLOAD
Download the new Media Selection Guide
DOWNLOAD
Download the Seahorse Plastics Brochure
DOWNLOAD

Supplier

Agilent Technologies, Inc.

VISIT WEBSITE

Request a demo or a quote

You control your cells. Not the other way around.

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

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