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Agilent Technologies, Inc.
Agilent Seahorse XFe24 Analyzer
Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Features
- Real-time Results – this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
- Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37°C).
- Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
- Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42°C (12°C-20°C above ambient conditions).
- Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
- Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
- Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
- Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
- Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
- Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
- Easily create assay protocols and analyze data with Seahorse Wave software
- For Research Use Only. Not for use in diagnostic procedures.
Technical specifications
Number of Assay Wells
20 - 22
Microchamber volume
7 µl
Controler
Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.
Software
Design and analyze assay templates on Controller or Wave desktop.
Best for
- Islets
- Larger samples
- small model organisms
Key Advantages
- Validated for hypoxia
- Validated for assay temperatures 16-42°C (12–20°C lower ambient temp required)
Plate Format
24-well microplate
All Agilent Seahorse XF Analyzers Feature
- Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
- Ability to perform up to 4 independent injections per well with automatic mixing
- Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
- Simultaneous measurement of OCR and ECAR in the same well
- Analytical Sensitivity for small sample sizes
- Label-free detection in real time
- Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data
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