header background-block header background-block header background-block header background-block

Find your product

Agilent Technologies, Inc.

Agilent Seahorse XFe96 Analyzer

Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

filler image

Features

  • Real-time Results – this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37°C).
  • Flexible Assay Design – the 96-well plate format accommodates many conditions in a single run and is best for dose-response studies and compound screening.
  • High Sensitivity – analyze as few as 5000 cells per well in the custom 96-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42°C (12°C-20°C above ambient conditions).
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
  • Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
  • Analyze 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate
  • Easily create assay protocols and analyze data with Seahorse Wave software

Technical specifications

Number of Assay Wells

92

Microchamber volume

2 µl

Controler

Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.

Software

Design and analyze assay templates on Controller or Wave desktop.

Best for

  • Phenotypic Screening
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids

Key Advantages

  • Maximum experimental flexibility
  • Highest throughput
  • Validated for hypoxia
  • Validated for assay temperatures 16-42°C (12–20°C lower ambient temp required)

Plate Format

96-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data

Multimedia

filler image
filler image
filler image

News & Events

News

Resources

Downloads

Download the Seahorse XF Instrument Selection Guide
DOWNLOAD
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
DOWNLOAD
Download the new Media Selection Guide
DOWNLOAD
Download the Seahorse Plastics Brochure
DOWNLOAD

Supplier

Agilent Technologies, Inc.

VISIT WEBSITE

Request a demo or a quote

The best super-resolution microscopy experience, on your desktop.

The industry’s first scalable, customizable, single-cell DNA analysis platform.

You control your cells. Not the other way around.

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format.

Seahorse XFp Analyzers measure OCR and ECAR of live cells in an 8-well miniplate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

The next generation single cell analysis system for antibody discovery and cell line development.

Simple Technology Platform with multiple Applications that is revolutionizing Protein Analysis

High Throughput Cell and Bead Based Analysis

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx™ Microbial Cell Counter automatically identifies and count individual bacterial cells

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

Arcturus Cellect Laser Capture Microdissection (LCM) System

DeepLabel™ Antibody Staining Kit for Cleared Tissues

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

N1 Single Cell Dispenser Sorting for 96 or 384-well plates

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Picodroplet Single Cell Encapsulation System and Picodroplet Single Cell Assay and Isolation System.

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Consumables for Research Instruments and other Microfluidic Systems

Total cell solution

Simple Technology Platform with multiple Applications that is revolutionizing Protein Analysis

Overview of Consumables for the XF96 and XF24 Analyzers

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

High Throughput Cell and Bead Based Analysis

Simultaneously measures the two major energy producing pathways in live cells

Optimized solution for assessing mitochondrial function.

Measures the capacity of the glycolytic pathway by driving cells toward glycolysis.

Provides real-time measurements of glycolysis

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

iQue Screener PLUS Consumables and Supplies

An Error occurred please try again later.
Please fill in all mandatory fields.

* All mandatory fields must be completed.

Thank you for contacting us!

{{ successMessage }}