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Agilent Technologies, Inc.

Agilent Seahorse XFp Analyzer

Seahorse XFp Analyzers measure OCR and ECAR of live cells in an 8-well miniplate format. The quick and easy setup makes the Seahorse XFp Analyzer ideal for performing routine measurements of ex-vivo metabolic phenotype and other quantity-limited samples.

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  • Right Size for Precious Samples – the XFp Analyzer is the most cost-effective solution for researchers with limited cell numbers or who have just a few samples to run each day.
  • Real-time Results – this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  The instrument performs measurements and calculates rates on the fly, completing the assay in just an hour or two. Data is easily transferred to Seahorse Wave analytical software, or exported to common spreadsheet and graphing programs.
  • Fast Turnaround Time – the 8-well plate miniplate format streamlines assay setup.  Custom report generators aid and standardize outputs for key assays while providing flexibility to support different experimental protocols.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing function.
  • High Sensitivity – analyze as few as 5000 cells per well/15000 per group.  Cell number requirements vary by cell type; consult the Cell Reference Database for details.
  • Compatible with a variety of sample sources due to the precision-controlled heating tray, which can maintain 16-40°C (minimum 8°C above ambient conditions).
  • Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure mitochondrial function with the Seahorse XFp Cell Mito Stress Test Kit and report generator.
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on glucose, glutamine, or fatty acids with the Seahorse XFp Mito Fuel Flex Test Kit and report generator.
  • Measure T cell activation with the use of programmable stimulant injection ports.

Technical specifications

Number of Assay Wells


Microchamber volume

2 µl


Integrated full-color touch-screen interface to design basic protocols and run imported templates.


Create and run standard assays on controller OR
design on Wave desktop, transfer to Controller, return to desktop for analysis.

Best for

  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Patient-derived samples

Key Advantages

  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (8–20°C lower ambient temp required)

Plate Format

8-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data


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News & Events




Download the Seahorse XF Instrument Selection Guide
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
Download the new Media Selection Guide
Download the Seahorse Plastics Brochure


Agilent Technologies, Inc.

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