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IntelliCyt, a Sartorius Brand

High Throughput Cell and Bead Based Analysis

Meet the new iQue3

The iQue Screener PLUS platform is an integrated instrument, software and reagent system that enables rapid, high content, multiplexed analysis of cells and beads in suspension.

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Meet the Intellicyt® iQue3

Faster, Smarter Flow Cytometry

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The new iQue3 is the most advanced flow cytometry platform with a focus on speed from setup, to acquisition, and analysis. To get you from samples to actionable results in record time.

The iQue3 combines a patented sampling method which allows for the fastest sample acquisition in the industry. It has the ability to handle 96, 384 well plates and enables continuous plate loading through connection with any automation system.

A new Enhanced Rinse Station (ERS) has been incorporated in the iQue3 which provides:

  • 48 hour uninterrupted run time before replacing reagent cartridges
  • Intelligent software monitoring of rinse station fluid levels
  • Warnings for missing, low, and even user defined volume levels
  • Automated vortexing just prior to sampling for improved Q.C. results
  • Improved reagent cartridges with less evaporation and the ability to easily close and store if needed.

Multiplex Phenotype and Function from Less Sample in Record Time

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The Intellicyt iQue Screener PLUS platform is an integrated instrument, software, and reagent system that enables simultaneous evaluation of cells and proteins using less sample while providing actionable multi-parametric data in record time.

While standard flow cytometry is commonly used to identify and characterize cells and beads in solution. It is has limitations in assay throughput, automated data analysis capabilities, sample/reagent volume requirements, and ease-of-use.

Speed to actionable results from every well

Delivering more of what matters when profiling cell function and phenotype.

Unlocking the secrets of immunology can be tough.

  • Are you developing a new immuno-oncology target assay…but struggling to get the right content with throughput?
  • Are you trying to profile lead candidates…but find your boss doesn’t want to foot the bill for it?
  • Are you developing CAR-T cells for patients and just need a fast way to functionally characterize the best cancer cell killers… but you have precious few cells to study, and time is short?
  • Are you monitoring a complex cytokine profile for sets of immuno-phenotyped PBMC’s… but making sense of all the data takes longer than running the experiment?

Plate readers are fast but can’t give you the individual cell measurements and multiplexing needed. Plate imagers produce high content data but don’t work well with suspension cells. Traditional flow cytometry is optimized for suspension cells but is slow, requires expert operators, consumes a lot of cells and reagents, and deals with the data as a collection of “tube experiments”. There must be a better way!

The Intellicyt iQue Screener PLUS platform addresses these limitations with these advantages:

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  • Speed
    Relieve capacity bottlenecks with the fastest plate sampling, integrated analysis, multiplexed no-wash assays, and novel data reduction tools.  
  • Miniaturization
    Conserve precious samples and reagents by sampling as little as a single microliter from miniaturized assay volumes.  
  • Content
    Perform high content, multiplexed analysis of cells, beads and secreted proteins in suspension.
  • Usability
    Enjoy a simple, scalable, multi-user environment with walkaway automation, comprehensive analysis and visualization tools, and other features not found in traditional solutions.  
  • Insight
    Dramatically reduce time to decision with novel visualizations that facilitate the fastest path to actionable results, even from complex data sets.

Rapid, Walkaway Plate Processing

  • Patented, rapid microvolume sampling processes 96 well plates in as few as 5 minutes or 384 well plates in 20 minutes.
  • Samples are delivered to detectors in an air-gap delimited stream, so an entire plate of data can be processed at one time.
  • Generous cartridge station allows for unattended multiple plate processing.
  • Interface and integration development kit allows easy use of your favorite automation.
  • Assay protocols are configurable for any point during or after plate sampling.
  • Perform routine tasks with the touch of a button, including system cleaning, plate map calibration, shutdown, and even fluidics refilling (with the QMax Refill Module).

Multiple Configurations Based on Your Need

The iQue3 comes in multiple optical configurations and flexible plate formats

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The easiest software you'll ever love!

  • Analyze a whole plate at a time as data is acquired not a "tube" at a time then batch analysis
  • One stop workflow. Get IC/EC50 curves, standard curves, derived outputs without 3rd party software
  • Easy assay optimization: adjust gates and see results across plate in real time
  • Plate level result visualizations with a mouse click then populated automatically
  • Condense multiplexed data sets into a single actionable results "map" automatically
  • Distributed access and reporting to make your organization more productive
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Create assays with validated reagents for cell function and secreted protein quantification.

  • Formulated for no wash to save time and reduced variability
  • Optimized for miniaturization on the iQue Screener PLUS  Platform to save costs and precious cells
  • Create high content data with less sample by multiplexing assays in same well
  • Combine immunophenotyping, cell health assessment, and cytokine profiling in same well
  • Preconfigured protein capture or build your own custom capture and multiplexed together
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Sofie Pattijn, CTO ImmunXperts

"In short, the iQue offers flow cytometry capabilities at real high throughput, making it the perfect instrument for cell and bead based screenings."

Andrea Gorlani, PhD, Scientist ModiQuest Research

"The iQue Screener is Changing the Way We Obtain Compelling Data"

“In-solution, cell-based assays are the way forward and the ability to do so with the iQue Screener in a 384-well high-throughput format has been extremely useful to us. We can now use one third of the materials and screen 4 times more antibody clones in the same amount of time, obtaining more compelling data for ourselves and our clients.”

Alison Tarke, Research Scientist, PersImmune

"The iQue Screener PLUS has greatly facilitated our work in the development of a personalized immunotherapy for AML and MDS patients."



IntelliCyt, a Sartorius Brand

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