Comprehensive Profiling with Single-Cell Resolution

Cellular heterogeneity is a key factor determinant in therapeutic response, resistance, relapse and residual disease. In order to increase therapy efficacy, the mutational content of patient samples and their effects on cellular composition must be determined.

In his talk entitled "Exosomes and super-resolution microscopy." Dr McNamara will present his development of a nanometer-scale workflow for the visualization of de novo synthesized exosomes, their purification from cell-free fluids, and how their cargo is trafficked in recipient cells. You will also have the opportunity to learn how the Nanoimager supports single-molecule imaging of extracellular vesicles.

Speaker:
Dr. Ryan McNamara
Translational Fellow at University of North Carolina at Chapel Hill

This webinar will outline the results of a study that performed integrative single-cell genome and cell surface protein expression profiling of adult acute myeloid leukemia (AML) cases. 

Genomic studies of AML have identified mutations with varying allele frequencies, which suggests a step-wise acquisition of mutation in leukemogenesis. Bulk sequencing can provide prognostic information, but it cannot delineate clonal architecture at the single-cell level. Additionally, the complementation of immunophenotype with corresponding mutational analysis could elucidate important dynamics in the leukemic cell population, but has yet to be assayed in parallel.

In this webinar, Linde Miles of Memorial Sloan Kettering Cancer Center will share data from the study demonstrating that simultaneous single-cell sequencing of DNA and protein can identify populations of stem/progenitor cells with distinct clonal/mutational and protein expression profiles. She will also discuss how the method identified trends in protein expression when comparing multiple samples with similar mutations. 

Attendees of this webinar will learn about:

• New DNA+protein capability from Mission Bio for simultaneous, targeted, single-cell DNA and protein sequencing
• A novel 6-antibody cocktail in combination with a single-cell targeted DNA custom AML panel 
• The relationship between mutational profile and cell surface protein expression in AML samples from six clinical research samples 

Speaker:
Linde A. Miles, PhD
Postdoctoral Research Fellow,
Memorial Sloan Kettering Cancer Center

Please join us for an exclusive webinar featuring Tracey Mullen, Chief Operating Officer, Abveris and AbX Biologics, who will review an antibody discovery campaign at Abveris targeting a glycoprotein for induction of apoptosis. Methods for immunization, screening for lead antibody candidates and subsequent functional characterization through the use of the Intellicyt® iQue platform will be detailed.

Who should attend:

• Antibody discovery scientists and researchers engaged in therapeutic drug discovery for oncology indications.
• Anyone interested in learning more about leveraging high-throughput flow cytometry analysis to advance research and development.