With this seminar, we aim to introduce you to latest developments and tools in biomarker research. The event will cover the broad range of biomarkers, from sub-cellular to live-cell markers.

We will highlight the impact of analyzing exosomes and microvesicles as well as virus-associated markers. Further you will learn more about the impact of metabolic switches and body-like microenvironments for primary cell culture.

Finally, biomarker aspects of single-cell cytokine profiles will be discussed as well as massive-parallel cytokine quantitation & phenotyping of cells.

Key topics to be covered

• Context on why and when mitochondrial toxicity should be screened for in early stages of drug development
• Clear cut-offs and rules are detailed
• Discussion on the results obtained with 23 reference drugs, including 4 pairs of of drugs which are structurally related but associated with different DILI concerns in human.

Mitochondrial toxicity is known to induce drug induced liver and cardiac injury, the main organ toxicities responsible for toxicity related attrition rates. Real-time cell-based bioenergetics assessment for mitochondrial toxicity can be part of the battery of safety screening assays in early drug development phases. This talk assesses the Seahorse XF platform as a tool for screening mitochondrial dysfunction and presents a workflow with reference to a panel of mitochondrial toxic drugs.

This webinar will discuss a study that used CRISPR/Cas9 to engineer mice harboring risk variants associated with glaucoma in order to assess their functional relevance. 

Dr. Skowronska-Krawczyk will discuss how her team used NanoCellect’s WOLF Cell Sorter to sort the RGCs required for this study. The live, purified RGCs, isolated from retinas three days after intraocular pressure elevation, were assessed for transcriptional and epigenetic changes in the context of the SIX6 genetic variant. Sample preparation steps and bioinformatic analysis of the findings will also be presented.

Speaker:
Dorota Skowronska-Krawczyk, PhD
Assistant Professor of Ophthalmology
University of California San Diego, Shiley Eye Institute