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Agilent Technologies, Inc.

NovoCyte® Advanteon™ Benchtop Flow Cytometer

Advancing the Boundaries of Flow Cytometry

The Agilent NovoCyte Advanteon flow cytometer builds upon the highly successful Agilent NovoCyte and NovoCyte Quanteon to provide an advanced set of capabilities for the most demanding scientists, yet is remarkably simple to operate. The NovoCyte Advanteon can accommodate today’s high-end and increasingly sophisticated multicolor flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors.

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NovoCyte Advanteon Flow Cytometer Features

The NovoCyte Advanteon can accommodate today’s high-end and increasingly sophisticated multicolor flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors. The NovoCyte Advanteon is customizable to meet your specific needs, and is easily upgradeable to meet your future demands. When throughput is essential, add Agilent NovoSampler Q to your laboratory automation platforms. It can efficiently process both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. For the NovoCyte Advanteon, the intuitive and industry-honored Agilent NovoExpress software is now even more advanced, providing an exceptional user experience in data acquisition, analysis, and reporting.

  • Expanded customization and upgrade potential with up to 21-color options using up to 3 lasers
  • Sample recovery mode serves to collect unused sample at end of acquisition
  • Superior sensitivity and resolution
  • Intuitive and powerful software for data acquisition, analysis, and reporting
  • Smart-design functionalities and walk-away operation simplifies your workflow
  • Automation-ready for high-throughput needs
  • Wide, 7.2-log dynamic range eliminates the need for routine detector adjustments
  • High-speed collection of up to 100,000 events/second
  • Accurate absolute cell count in every experiment, which eliminates the need for reference beads

The Ultimate Photodetector

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Silicon photomultipliers (SiPM) are solid-state, photon-level sensitive semiconductor devices. The innovative optical design with the NovoCyte Advanteon incorporates 21 independent SiPM, which collect light on each and every channel, with sensitivities that far exceed the traditional PMT’s.

SiPM's strengths:

  • Superior photon detection sensitivity
  • High gain and high quantum efficiency
  • Instantaneous warm up and fast response
  • Robust and long lifespan
  • High durability

Consistent results, fast or slow

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The fluidic feedback control mechanisms found on the NovoCyte Advanteon consistently maintain exceptionally steady flow rates. The superior stability across a wide range of sample flow rates provide consistent results under variable operating conditions. As shown below, the CV’s at different flow rates are dramatically improved over other systems on the market.

Advantage of Performance and Flexibility

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With up to 21 fluorescence channels, the NovoCyte Advanteon offers tremendous flexibility in panel design, thereby allowing more data to be collected per run. Assessment of T cell state and function is just one critical example for researching cell-based immunotherapies using multi-color staining panels. Here, the NovoCyte Advanteon was used to show T cell differentiation, monitor T cell exhaustion, and measure cytokine proliferation from PBMC’s following activation, all in one experiment.



Agilent Technologies, Inc.

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Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

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