header background-block header background-block header background-block header background-block

Find your product

Back to Products

Agilent Technologies, Inc.

NovoCyte Opteon Spectral Flow Cytometer

The NovoCyte Opteon spectral flow cytometer is a new, cutting-edge spectral flow cytometry solution designed to revolutionize your cell analysis research.

The NovoCyte Opteon boasts up to 5 lasers and 73 detectors, a proprietary optics design, and advanced electronics, enabling it to deliver data with high resolution and sensitivity. The wide dynamic range for both fluorescence detection and particle size measurement helps streamline experimental workflows. On-board temperature control, electronics, and fluidics sensor circuitry provide real-time instrument status monitoring and ensure consistent, reliable data acquisition in different ambient environments.

Additionally, the intuitive and industry-leading NovoExpress software has been further advanced, providing an exceptional data acquisition, analysis, and reporting user experience.

filler image
filler image

NovoCyte Opteon Spectral Flow Cytometer Features

  • Up to 5 lasers, 73 high-quality detectors
  • Demonstrated panel of 45 colors
  • Data with high sensitivity and high resolution due to an innovative optical design
  • Dual-laser small particle detection with wide dynamic range
  • High instrument reliability with on-board temperature control and fluidics circuitry
  • Powerful and intuitive NovoExpress software
  • Versatile autosampler compatible with 40-tube rack and 384/96/48/24 well plates
  • Robotic automation ready

Every Fluorochrome Tells a Story

filler image

Armed with up to 5 lasers, 73 detectors, and a demonstrated 45-color panel, the NovoCyte Opteon is your gateway to multidimensional cell analysis. Expand beyond traditional flow cytometry panels to reveal more about your samples.

Elevate Your Data

filler image

Autofluorescence subtraction allows you to achieve improved resolution of specific cell populations of interest. Apply autofluorescence subtraction to individual cell populations in a mixed cell sample, allowing for customized resolution and improved sensitivity for your analysis.​

Smart Technology, Simple Solutions

filler image

The intuitive and industry-leading NovoExpress software sets a new standard for an exceptional user experience in flow data acquisition, analysis, and reporting. With the NovoSampler S, you’ll have true walk-away functionality that is ready for robotic automation that seamlessly integrates into your laboratory instrument ecosystem.

Applications

Immunophenotyping

filler image

There is a significant need for methodologies and instruments that provide high-throughput, in-depth analyses of the immune system at the single-cell level. Contrary to conventional cytometry, which is limited to as many fluorescence parameters as there are detectors, spectral cytometry is able to capture the full fluorescence spectrum for every marker across all laser lines, allowing for more parameters in a single experiment.

Small Particle Detection

filler image

The NovoCyte Opteon FSC/SSC detection optics and signal processing electronics have been optimized with dual 488 nm-SSC and 405 nm-SSC to resolve particles as small as 80 nm without adjusting settings to view larger cells in the same sample. With this, high-resolution platelets, bacteria, and various sub-micron particles can be readily identified and analyzed alongside your cell subsets.

Dual SSC to Separate RBCs from WBCs

filler image

The optimized dual 488 nm-SSC and 405 nm-SSC on the NovoCyte Opteon can be used to separate red blood cells (RBCs) from white blood cells (WBCs) and platelets without the need for any RBC lysis treatment.

Testimonials

Matthias Schiemann, Technische Universität München

“Software is straightforward, and the software interface is easy to handle”

“This (NovoExpress®) software is straightforward, and the software interface is easy to handle. The implemented auto compensation and hierarchic tree structure is a highlight for effective organization of experimental data.”

Resources

NovoCyte Opteon Spectral Flow Cytometer Video

Introducing the NovoCyte Opteon – the cutting-edge spectral flow cytometry solution designed to revolutionize your cell analysis research.

filler image

Supplier

Agilent Technologies, Inc.

Visit Suppliers Website

Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

An Error occurred please try again later.
Please fill in all mandatory fields.

* All mandatory fields must be completed.

Thank you for contacting us!

{{ successMessage }}