header background-block header background-block header background-block header background-block

Find your product

Agilent Technologies, Inc.

Seahorse XF Hu T Cell Activation Assay Kit

Agilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand to support effective proliferation, size expansion, and differentiation. Such principle is supported by recent advances in the field of immunology that recognize cellular metabolism as a primary driver and regulator of immune cell function and lineage commitment.

The kit contains Agilent-validated ImmunoCult Human CD3/CD28 T Cell Activator and 2-deoxy-D-glucose (2-DG) and is an optimal solution delivered by Agilent and STEMCELL Technologies partnership for interrogating and modulating T cell activation on the Seahorse XF platform. The ImmunoCult Human CD3/CD28 T Cell Activator from STEMCELL Technologies is a soluble tetrameric complex. It is easy-to-use and compatible with specially designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability. Together with standardized protocols and cloud-based Agilent Seahorse Analytics software, the XF Hu T Cell Activation Assay Kit provides a robust measure of T cell activation with new insights.

filler image
filler image


  • Detects human T cell activation in minutes, providing an early window to investigate cellular and signaling requirements associated with T cell activation
  • Provides kinetic readout for continuous monitoring of human T cell activation and modulation
  • Allows dosing of cells with test compounds/modulators during assay through the built-in injection ports
  • The soluble ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies) is easy-to-use and compatible with specially-designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability
  • Reagents are directly reconstituted or diluted in the assay medium, eliminating the need for DMSO or other solvents
  • Every lot is tested with functional assays to deliver consistent and robust results
  • Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF T Cell Activation assay, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs

How it works

filler image

A rapid early increase in glycolysis as a signature event for T cell activation

Antigen presenting cell (APC) or CD3/CD28 activators, upon recognizing and binding to T cell receptors, initiate a number of signaling cascades that lead a rapid increase in glycolysis and subsequent T cell proliferation, cytokine production and effector functions. It is recognized that the rapid early onset of glycolysis is a signature event of T cell activation and is required for subsequent T cell functions.

filler image

Seahorse XF technology offers a robust method for detecting T cell activation

The Agilent Seahorse XF Hu T Cell Activation Assay measures T cell activation response by monitoring glycolysis changes induced by activators. This rapid assay presents an early window to discriminate activation responses and provides kinetic functional information that typically cannot be obtained by orthogonal methods measuring gene expression, cell proliferation, or cytokine production. With standardized protocols and validated reagent, the XF Hu T Cell Activation Assay is a robust solution for studying T cell activation, modulation and related metabolic reprogramming.

filler image

Easy-to-use turnkey solution

The XF T Cell Activation Assay is a turnkey solution with standardized protocols, ready-to-use XF PDL cell culture microplates and streamlined data analytic tool. The standardized protocols support two experimental designs that allow for testing/comparing activation potential among experimental groups and for investigating acute effects of modulators. Organized/shareable summary reports are automatically generated by Agilent Seahorse Analytics. Precoated XF PDL cell culture microplates provide convenience and redcuce data variation commonly caused by manual coating processes.



Download the Agilent Seahorse XF Assay Kits & Reagents Overview
Download the new Media Selection Guide
Download the Seahorse Plastics Brochure
Download the Seahorse XF Instrument Selection Guide


Agilent Technologies, Inc.

Visit Suppliers Website

Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

An Error occurred please try again later.
Please fill in all mandatory fields.

* All mandatory fields must be completed.

Thank you for contacting us!

{{ successMessage }}