Agilent Technologies, Inc.
Seahorse XF Hu T Cell Activation Assay Kit
Agilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand to support effective proliferation, size expansion, and differentiation. Such principle is supported by recent advances in the field of immunology that recognize cellular metabolism as a primary driver and regulator of immune cell function and lineage commitment.
The kit contains Agilent-validated ImmunoCult Human CD3/CD28 T Cell Activator and 2-deoxy-D-glucose (2-DG) and is an optimal solution delivered by Agilent and STEMCELL Technologies partnership for interrogating and modulating T cell activation on the Seahorse XF platform. The ImmunoCult Human CD3/CD28 T Cell Activator from STEMCELL Technologies is a soluble tetrameric complex. It is easy-to-use and compatible with specially designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability. Together with standardized protocols and cloud-based Agilent Seahorse Analytics software, the XF Hu T Cell Activation Assay Kit provides a robust measure of T cell activation with new insights.
Features
- Detects human T cell activation in minutes, providing an early window to investigate cellular and signaling requirements associated with T cell activation
- Provides kinetic readout for continuous monitoring of human T cell activation and modulation
- Allows dosing of cells with test compounds/modulators during assay through the built-in injection ports
- The soluble ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies) is easy-to-use and compatible with specially-designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability
- Reagents are directly reconstituted or diluted in the assay medium, eliminating the need for DMSO or other solvents
- Every lot is tested with functional assays to deliver consistent and robust results
- Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF T Cell Activation assay, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
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Agilent Technologies, Inc.
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