Agilent Technologies, Inc.
Seahorse XF T Cell Metabolic Profiling Kit
The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or avoid exhaustion in the tumor microenvironment. The kit not only provides improved reagents, but also features a streamlined assay workflow, reducing assay preparation time and minimizing the need for uncoupler optimization. The XF T Cell Metabolic Profiling kit is also integrated with Wave Pro and Seahorse Analytics software to simplify data analysis, visualization, and interpretation.
Each assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF Pro and XFe/XF96 analyzers. The XFp kit is for use with the Agilent Seahorse HS Mini and XFp analyzers.
Features
- Supports two assays for generating robust bioenergetic parameters linked to critical attributes for antitumor properties: T cell persistence and T cell metabolic fitness
- Provide a comprehensive view of T cell energy metabolism
- Suitable for evaluation of different construct designs, engineering strategies, starting material selection, or metabolic conditioning during the cell expansion process
- Applicable for use in assessing the capacity of T cells to maintain metabolic fitness in metabolically restrictive conditions (e.g., tumor microenvironment)
- Improved reagents to minimize optimization of uncouplers for different T cell populations
- Validated for and best performed with Agilent Seahorse XF Pro analyzer, XF HS mini analyzer, XF RPMI medium, pH 7.4 (Cat. No. 103576-100) and supplements (Cat. No. 103577-100, 103578-100, 103579-100)
- Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
- Every lot is rigorously tested and validated with functional assays to deliver consistent and robust results
- Easy-to-interpret data analysis provided by the Agilent Seahorse Analytics, a cloud-based platform, simplifying data processing and transforming raw data into organized, shareable summary reports
- Use this link to access Agilent Seahorse Analytics for data analysis: https://seahorseanalytics.agilent.com
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Agilent Technologies, Inc.
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