header background-block header background-block header background-block header background-block

Find your product

Bionano

Ionic® – The Next-Gen Sample Prep System

The revolutionary Ionic® Purification System requires no binding, no stripping, and no washing from fixed surfaces for much higher yields and higher quality nucleic acid.

filler image

A Superior Automated Approach to DNA and RNA Extraction

The Ionic Purification System is so different, its advantages are most readily understood in contrast with conventional DNA and RNA extraction and purification methods.

  • No organic solvents
  • No beads or repetitive washing
  • No harsh, high-salt buffers
  • No hands-on mixing, separation, sample transfers, or buffer exchanges
  • No system programming
  • No pumps, valves, or other moving parts

Ionic Purification System

filler image

The Ionic Purification System uses Isotachophoresis (ITP)
to extract, purify, and concentrate genomic DNA and RNA
from cells and FFPE. DNA and RNA samples prepared
by the system are pure, abundant, and ready in just 60 minutes.

Learn more about isotachophoresis
filler image

Want Higher Amplifiable Yields?

Extract Up to 50% More Nucleic Acid from Cells and FFPE

qPCR results from the amplification of a 280bp amplicon in DNA purified from cell populations ranging from 1,120 to 56,000 cells demonstrate that purification on the Ionic system results in values that are ~3 Ct lower than those from a column-based method. For populations with fewer cells, some eluates from the column-based competitor do not contain detectable amounts of DNA.

filler image

Achieve Yields of 3.5x or More from FFPE Samples

Results for 32 FFPE block samples* purified from scrolls for Purigen (no paraffin removal) vs. slides (excess paraffin removed) for a column-based approach. Yields for Purigen samples demonstrate improved amplifiable yields of on average 3.5x versus column-based approach.

Purigen SCROLLS vs. Column-based Kit SLIDES

filler image

Want Higher Quality Results?

More Uniform Coverage

NGS results of DNA purified from FFPE samples analyzed using the Agilent SureMASTR Tumor Hotspot sequencing panel with 252 amplicons ranging from 128 bps to 245 bps show that samples purified on the Purigen Ionic system have tighter clustering when compared to samples prepared using a column-based method – indicative of more uniform sequence coverage.

filler image

No Bias Toward Length or GC Content of Amplicons

Taking a closer look at the results from above shows that DNA from FFPE samples purified on the Ionic system is less biased toward GC content and fragment length than from FFPE samples purified using a column-based method.

filler image

*Data for the graphs above is normalized by a reference sample to remove the effect of coverage differences introduced by the amplification efficiency of the target amplicon. Data from the reference sample incorporates purification results from the Purigen system and a column-based method to avoid biasing the reference sample toward either technology.

Multimedia

News & Events

News

Upcoming Events

Resources

Purigen Isotachophoresis for Pure DNA and RNA Extraction

A Superior Approach to Nucleic Acid Purification

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Bionano Ionic® Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there is no binding to, or stripping from, fixed surfaces. The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination.

Learn more about Isotachophoresis

Supplier

Bionano Genomics, Inc.

Visit Suppliers Website

Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

Complete Characterization of Exosomes and Extracellular Vesicles (EVs)

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single cells or nuclei from solid tissues in minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Overview of Consumables for the XF96 and XF24 Analyzers

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Simultaneously measures the two major energy producing pathways in live cells

Optimized solution for assessing mitochondrial function.

Measures the capacity of the glycolytic pathway by driving cells toward glycolysis.

Provides real-time measurements of glycolysis

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

An Error occurred please try again later.
Please fill in all mandatory fields.

* All mandatory fields must be completed.

Thank you for contacting us!

{{ successMessage }}