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NanoCellect® Biomedical, Inc.

Wolf® G2 Cell Sorter

Healthy Cells. Broad Capabilities. Better Science.

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing.

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Wolf G2 Cell Sorter Features

The WOLF was originally created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells effectively, easily, and of high quality. Now, as science continues to move forward into increasingly complex realms, the WOLF is moving forward with it.

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing.

Single-cell sorting can be completed in 96- or 384-well plates when using the WOLF G2 in conjunction with the N1 Single-Cell Dispenser. This flexibility in performance, along with the additional abilities of the second laser, makes it ideal for use in many different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, immunology, infectious disease, basic research, and more.

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  • SMALL: At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.
  • GOES ANYWHERE: The WOLF G2 is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets.
  • INTUITIVE SOFTWARE: Unlike traditional cell sorter software, easy to use and requires minimal training.
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At < 2 psi, the WOLF G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells.

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All laser configurations afford < 250 MESF sensitivity, along with forward and back scatter, providing as low as 1 μm resolution.

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At 2 cubic feet, NanoCellect’s benchmark for access and performance allows every lab for the flexibility to do analysis and sorting into tubes or 96- and 384-well plates.

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Intuitive software, fixed optics, no fluidics cart and less than one minute clean-up time.

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Disposable, aerosol-free microfluidic cartridge allows for sterile sorting that protects the sample from the environment and scientist from the sample.

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With two lasers and up to nine fluorescent channels, the WOLF G2 aligns with a broad set of research applications and experiments. Three different laser configurations allows options specific to your needs.

Catridge-based Fluidics

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NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.

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Product Specs Table Optics

The WOLF G2 uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF G2 utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 different laser configurations to broaden its capabilities. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.

Available Laser Configurations:

  • 488 nm & 637 nm
  • 488 nm & 561 nm
  • 405 nm & 488 nm

Software

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  • WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
  • After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
  • Key tools like automatic compensation and index sorting, are streamlined and included.
  • When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
  • Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.

Performance

The WOLF G2’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF G2 can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 μm, and up to 60 μm. Users can expect a sorting speed of up to 200 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.

The power of modern and simple cell sorting in many applications

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Genomics

Sample preparation is a significant yet underappreciated step that allows for high quality sequencing and analysis, critical to achieving breakthroughs in medicine, agriculture, and other industries. NanoCellect’s microfluidic cell sorter, the WOLF, allow the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab, make it a key step to any cell sequencing analysis workflow.

  • 10x Genomics RNA-seq library prep
  • Single-Cell sorting on PCR plate
  • Whole-genome sequencing methods
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Antibody Discovery

The antibody drug discovery process differs from the traditional drug discovery of small molecules as it requires the development of hybridomas and the engineering of antibodies to recognize a specific target protein. The production of recombinant antibodies in cancer treatment as well as the development of neutralizing antibodies for infectious diseases, has allowed antibody discovery platforms to interrogate the immune repertoire and develop novel antibody libraries using flow cytometry analysis and cell sorting. The WOLF cell sorter provides a simple, gently cell sorting solution for many workflows such as:

  • Antibody Discovery from Single Bovine B-Cells
  • Isolate Antibody-Secreting CHO Cells
  • Recombinant protein production in CHO Cells
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Cell Line Development

Generating cell lines has often been inefficient and affected by inaccurate limited dilution methods or the shear stress caused by conventional flow cytometer and traditional high-pressure cell sorters. NanoCellect Biomedical provides cell sorting solutions that allow to sort cells and establish stable cell lines using gentle low-pressure microfluidics. The WOLF’s ability to protect cell viability after sorting makes it an integral part of any cell line development workflow such as:

  • Recombinant protein production in CHO Cells
  • Sorting stem cell cultures
  • Antibody discovery
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Gene Editing

Isolating edited-pluripotent stem cells with traditional sorting techniques can subject the cells to rough conditions in which gene expression, genomic stability and overall cell-health can become compromised. Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. Learn more about how cell sorting can improve workflows such as:

  • CRISPR and disease modelling
  • Protection of Karyotype
  • Generation of Genome-Edited iPSCs
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N1 Single Cell Dispenser

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  • Quickly and effortlessly plate your cells into 96 or 384 well plates.
  • Output flexibility allows users to dispense 1 or “n” cells per well.
  • Excellent for single-cell cloning or single-cell RNA-seq.
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Testimonial

Prof. Dr. Angela Ciuffi, Univ. de Lausanne, Faculté de Biologie et Médecine

"The powerful combination of flow cytometric analysis, sorting and single cell dispensing in an intuitive and easy-to-use device as the Wolf Cell Sorter by NanoCellect allows for affordable, high-quality and reliable analyses and experiments."

"The disposable single-use cartridges ensures the safe analysis of infected samples as well as the absence of sample cross-contamination without the need of time-consuming extensive washing of the equipment."

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NanoCellect Biomedical Inc.

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