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Nanoimager – The best super-resolution microscopy experience, on your desktop!

Super-Resolution in many forms

The Nanoimager offers various modes of operation including dSTORM, PALM, smFRET and supports illumination modes from epifluorescence to TIRF. With this range on offer, it's easier than ever to get the most out of fluorescence microscopy, both when imaging fixed samples stained with immunofluorescence protocols or during live-cell imaging.

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Imaging techniques and illumination modes

Super-Resolution in many forms

The Nanoimager offers various modes of operation including dSTORM, PALM, smFRET and supports illumination modes from epifluorescence to TIRF. With this range on offer, it's easier than ever to get the most out of fluorescence microscopy, both when imaging fixed samples stained with immunofluorescence protocols or during live-cell imaging.

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  • Single-Particle Tracking
    Microfluidics compatible
    Whole body heating
    • Dedicated tracking analysis
    • Resolution up to 20 nm
    • Real time rendering
    • 3D imaging
  • Single-Molecule FRET
    • Interactions within 1-10 nm range
    • Individual and group events
    • Dedicated smFRET analysis

Super-Resolution Microscopy Applications

The next generation of super-resolution microscope reveals biology in a new light. We can now image cells at the highest resolution, understand molecular interactions and dynamics and study the fundamentals of biology through single-molecule fluorescence.

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Extracellular Vesicles Hub

Extracellular vesicles (EVs) play key roles in cell-to-cell communication. EVs can cross biological barriers (such as the blood-brain barrier) and get internalized into the cell with a high degree of specificity.

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Pathogens Hub

Infectious diseases are caused by pathogenic microorganisms such as bacteria, viruses, fungi or prions. Super-resolution microscopy helps find more efficient ways of diagnosing, treating and preventing them.

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Other Case Studies

Biosafety Cabinets

Until recently, due to their size and design, the majority of super-resolution microscopes have not been able to support research in enclosed, ventilated biosafety cabinets. But things are changing.

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Tracking Extracellular Vesicles

Visualize and track EVs in solution or within cells to understand their behaviour.

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Tracking Viral Particles

Understanding and quantifying viral particle behavior.

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Viral Particle Imaging

Viral particles vary greatly in size, but are typically below the resolution limit of conventional light microscopy. Recently, super-resolution techniques have been employed to study their mechanistic and functional characteristics at a single-molecule level.

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Host Pathogen Interactions

By helping researchers to unravel new, dynamic information about virus particles, super-resolution micoscopy enables breakthroughs in the understanding of viral pathogens and in finding new targets to treat viral infection.

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Visualizing Extracellular Vesicles

Super-resolution imaging can be used to study fine morphological details and precise localization of EV-associated proteins.

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Tracking Single Molecules and Vesicles in cells

The Nanoimager can track single molecules and vesicles in both bacterial and eukaryotic cells with super-resolution microscopy.

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Quantitative Cellular Imaging

Gain detailed understanding of cellular features through multi-color super-resolution microscopy with advanced data analysis including colocalization and clustering.

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Protein Complex Assembly

Intensity measurements, super-resolution and multi-color labeling for characterizing protein complexes and their assembly.

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Molecular Mechanisms and Interactions

Using smFRET to understand molecular interactions and dynamics, including enzymes and substrates, protein aggregates in neurodegeneration and other protein-protein interactions.

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DNA Conformational Changes

A dynamic, real-time nanoscale ruler, now a general tool for characterizing molecular interactions and structure with Alternating Laser Excitation (ALEX) support.

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Epigenetic Mapping

Imaging specific target sites on DNA at the single-molecule level: a simple tool for characterizing the distribution of epigentic sites.

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DNA-PAINT shows sub-20nm resolution

DNA-PAINT provides easy localization-based super-resolution with nanorulers from GATTAquant GmbH.

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Nanoimager Features

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  • Microfluidics compatible
  • Temperature Control to 42℃
  • Drift Correction
  • Real-time Autofocus
  • High Laser Power Density
  • Unlimited Licences for Analysis Software
  • Class 1 Laser Product
  • LED Array for Brightfield Illumination
  • Piezo Stage with 2nm Step size


News & Events

On-Demand Webinar

Bucher Biotec Webinar on

Exosomes – Carriers of future Diagnostics and Therapeutics

Multi-level visual Characterization of Exosomes and other Extracellular Vesicles

Extracellular vesicles (EVs), such as exosomes, are nano-sized particles secreted by all cell types. Because of their ability to transfer a wide variety of biological cargo from donor to recipient cells, EVs have the potential to exert potent effects on target cells or serve as potential biomarkers in disease detection.

In this joint-webinar with NanoView Biosciences and ONI you will learn about the importance of precise and detailed visual characterization of single EVs and exosomes using state-of-the-art, cutting-edge technology.

Alex Shephard, Ph.D.
Senior Application Scientist at NanoView Biosciences

Ricardo Nunes Bastos, Ph.D.
Director Business Development at ONI

  • 7. December 2021, 10:30 AM

ONI Webinar on

Harness the power of single-molecule detection, at your finger tips

Dr. Siobhan King presents a talk entitled, "Harness the power of single-molecule detection at your finger tips". She highlights applications that can be performed on the Nanoimager including detecting and visualizing extracellular vesicles as well as imaging and tracking viral particles.

ONI Webinar on

A journey through the cell: Unveiling life at a single-molecule level

Dr. Harmon presents a talk entitled, "A Journey through the Cell - Unveiling life at a single-molecule level". He highlights how to visualize structures from cell membrane to nucleus in unprecedented detail. With its small but mighty design, the Nanoimager delivers the ability to see, count and track single molecules from the convenience of your benchtop.


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Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

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