header background-block header background-block header background-block header background-block

Find your product

Agilent Technologies, Inc.

Agilent Seahorse XF HS Mini

Agilent Seahorse XF HS Mini Analyzers measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells in an eight-well miniplate format. The quick and easy setup makes the Seahorse XF HS Mini Analyzer ideal for performing routine energy metabolism measurements of ex-vivo and other quantity-limited samples.

filler image

Features

  • Analyzer is compatible with XFp miniplates and XFp PDL miniplates for routine pairwise comparisons.
  • Reports oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in minutes, without sample extraction or labeling.
  • Four-port injection system with automated mixing function enables detection of live-cell responses to substrates, activators, inhibitors, and other compounds in real time.
  • Precision-controlled heating tray maintains sample at 16–40 °C (minimum 8 °C above ambient conditions) for compatibility with a variety of sample sources.
  • Cloud-based Seahorse Analytics provides accessible, streamlined tools to interpret your data.

Technical specifications

Number of Assay Wells

6

Microchamber volume

2 µl

Controler

Integrated full-color touch-screen interface to design basic protocols and run imported templates.

Data Analysis

Seahorse Analytics; a web-based accessible platform with integrated assay parameter calculators

Best for

  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Primary cells and ex-vivo samples

Key Advantages

  •  
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (8–20°C lower ambient temp required)
  •  

Plate Format

8-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Ability to measure mitochondrial function and rate of glycolysis in live cells
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Seahorse Analytics; web-based data analysis & collaboration software platform

Multimedia

filler image

News & Events

News

Webinar

BBT Webinar (recorded on 5. March 2024) on

Live Cell Metabolic Profiling, the Potential of Agilent Seahorse Analyzers and Assays

Join us for the second instalment of our webinar series, focusing on the dynamic world of live cell metabolic profiling using Agilent Seahorse Analyzers and Assays. Building upon our previous Luna Cell Counters webinar, we'll guide you through the potential of Agilent Seahorse Analyzers, providing insights into unraveling the intricacies of metabolic processes within live cells. Discover how these advanced tools not only provide valuable insights into cellular energetics but also offer a streamlined approach, saving you time and resources. Don't miss the opportunity to explore the forefront of live cell metabolic profiling with Agilent Seahorse, presented by experts in the field.

Resources

Downloads

Download the Seahorse XF Instrument Selection Guide
DOWNLOAD
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
DOWNLOAD
Download the new Media Selection Guide
DOWNLOAD
Download the Seahorse Plastics Brochure
DOWNLOAD

Supplier

Agilent Technologies, Inc.

Visit Suppliers Website

Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single cells or nuclei from solid tissues in minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

An Error occurred please try again later.
Please fill in all mandatory fields.

* All mandatory fields must be completed.

Thank you for contacting us!

{{ successMessage }}