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Agilent Technologies, Inc.

Agilent Seahorse XF HS Mini

Agilent Seahorse XF HS Mini Analyzers measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells in an eight-well miniplate format. The quick and easy setup makes the Seahorse XF HS Mini Analyzer ideal for performing routine energy metabolism measurements of ex-vivo and other quantity-limited samples.

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  • Analyzer is compatible with XFp miniplates and XFp PDL miniplates for routine pairwise comparisons.
  • Reports oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in minutes, without sample extraction or labeling.
  • Four-port injection system with automated mixing function enables detection of live-cell responses to substrates, activators, inhibitors, and other compounds in real time.
  • Precision-controlled heating tray maintains sample at 16–40 °C (minimum 8 °C above ambient conditions) for compatibility with a variety of sample sources.
  • Cloud-based Seahorse Analytics provides accessible, streamlined tools to interpret your data.

Technical specifications

Number of Assay Wells


Microchamber volume

2 µl


Integrated full-color touch-screen interface to design basic protocols and run imported templates.

Data Analysis

Seahorse Analytics; a web-based accessible platform with integrated assay parameter calculators

Best for

  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Primary cells and ex-vivo samples

Key Advantages

  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (8–20°C lower ambient temp required)

Plate Format

8-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Ability to measure mitochondrial function and rate of glycolysis in live cells
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Seahorse Analytics; web-based data analysis & collaboration software platform


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News & Events



BBT Webinar (recorded on 5. March 2024) on

Live Cell Metabolic Profiling, the Potential of Agilent Seahorse Analyzers and Assays

Join us for the second instalment of our webinar series, focusing on the dynamic world of live cell metabolic profiling using Agilent Seahorse Analyzers and Assays. Building upon our previous Luna Cell Counters webinar, we'll guide you through the potential of Agilent Seahorse Analyzers, providing insights into unraveling the intricacies of metabolic processes within live cells. Discover how these advanced tools not only provide valuable insights into cellular energetics but also offer a streamlined approach, saving you time and resources. Don't miss the opportunity to explore the forefront of live cell metabolic profiling with Agilent Seahorse, presented by experts in the field.



Download the Seahorse XF Instrument Selection Guide
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
Download the new Media Selection Guide
Download the Seahorse Plastics Brochure


Agilent Technologies, Inc.

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Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

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