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Agilent Technologies, Inc.

Agilent Seahorse XF HS Mini

Agilent Seahorse XF HS Mini Analyzers measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells in an eight-well miniplate format. The quick and easy setup makes the Seahorse XF HS Mini Analyzer ideal for performing routine energy metabolism measurements of ex-vivo and other quantity-limited samples.

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Features

  • Analyzer is compatible with XFp miniplates and XFp PDL miniplates for routine pairwise comparisons.
  • Reports oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in minutes, without sample extraction or labeling.
  • Four-port injection system with automated mixing function enables detection of live-cell responses to substrates, activators, inhibitors, and other compounds in real time.
  • Precision-controlled heating tray maintains sample at 16–40 °C (minimum 8 °C above ambient conditions) for compatibility with a variety of sample sources.
  • Cloud-based Seahorse Analytics provides accessible, streamlined tools to interpret your data.

Technical specifications

Number of Assay Wells

6

Microchamber volume

2 µl

Controler

Integrated full-color touch-screen interface to design basic protocols and run imported templates.

Data Analysis

Seahorse Analytics; a web-based accessible platform with integrated assay parameter calculators

Best for

  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Primary cells and ex-vivo samples

Key Advantages

  •  
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (8–20°C lower ambient temp required)
  •  

Plate Format

8-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Ability to measure mitochondrial function and rate of glycolysis in live cells
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Seahorse Analytics; web-based data analysis & collaboration software platform

Multimedia

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News & Events

News

Resources

Downloads

Download the Seahorse XF Instrument Selection Guide
DOWNLOAD
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
DOWNLOAD
Download the new Media Selection Guide
DOWNLOAD
Download the Seahorse Plastics Brochure
DOWNLOAD

Supplier

Agilent Technologies, Inc.

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