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Agilent Technologies, Inc.

Agilent Seahorse XF Pro Analyzer

Agilent Seahorse XF Pro Analyzers measure the oxygen consumption (OCR) and extracellular acidification rate (ECAR/PER) of live cells in a 96-well format. The XF Pro Analyzer features better OCR precision at low rates, verified instrument performance and repeatability specifications, optimized temperature control, and is automation enabled.

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Features

  • Better precision at low OCR allows for the confident interrogation of additional immune cell types and cell types that are bioenergetically compromised
  • Verified instrument performance delivers verified CV% for mid-high OCR/PER rate ranges, ensuring consistent XF data from plate to plate
  • Reports real-time metabolic rates in minutes, without sample extraction or labeling
  • Four-port injection system with automated mixing function allows detection of live-cell responses to substrates, inhibitors, and other compounds in real time
  • Achieve high-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
  • Precision-controlled heating tray maintains 16–42°C temperatures (8–20°C above ambient conditions) for compatibility with a variety of sample types
  • XF Pro Controller software allows easy assay protocol creation and user guided interface for performing XF assays. Wave Pro Data Quality View automatically flags erroneous data and allows for easier rejection of outlier data points.

Technology

 

Automatic measurement of energy metabolism in real time

Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

 

Discover the Power of Cellular Metabolism

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The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. Discover how bioenergetic parameters can provide powerful insights and can serve as an indicator for disease models, critical cell processes, and therapeutic discoveries.

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Immune Metabolism

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Immune cell processes including activation, proliferation, and memory development are all driven by metabolic reprogramming, which can be tuned to enhance performance and control immune cell outcomes. Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements.

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Cancer Metabolism

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Metabolism is a critical driver of cancer malignancy, with cancer cell proliferation commonly requiring an upregulation or “metabolic switch” towards a more glycolytic phenotype to fuel the rapid energy requirements and generate building blocks for cancer cell growth. Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology.

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Multimedia

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News & Events

News

Resources

Downloads

Download the Seahorse XF Instrument Selection Guide
DOWNLOAD
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
DOWNLOAD
Download the new Media Selection Guide
DOWNLOAD
Download the Seahorse Plastics Brochure
DOWNLOAD

Supplier

Agilent Technologies, Inc.

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