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Agilent Technologies, Inc.

Agilent Seahorse XFe24 Analyzer

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

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  • Real-time Results – this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37°C).
  • Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42°C (12°C-20°C above ambient conditions).
  • Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
  • Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
  • Easily create assay protocols and analyze data with Seahorse Wave software
  • For Research Use Only. Not for use in diagnostic procedures.

Technical specifications

Number of Assay Wells

20 - 22

Microchamber volume

7 µl


Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.


Design and analyze assay templates on Controller or Wave desktop.

Best for

  • Islets
  • Larger samples
  • small model organisms

Key Advantages

  • Validated for hypoxia
  • Validated for assay temperatures 16-42°C (12–20°C lower ambient temp required)

Plate Format

24-well microplate

All Agilent Seahorse XF Analyzers Feature

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data


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News & Events



BBT Webinar (recorded on 5. March 2024) on

Live Cell Metabolic Profiling, the Potential of Agilent Seahorse Analyzers and Assays

Join us for the second instalment of our webinar series, focusing on the dynamic world of live cell metabolic profiling using Agilent Seahorse Analyzers and Assays. Building upon our previous Luna Cell Counters webinar, we'll guide you through the potential of Agilent Seahorse Analyzers, providing insights into unraveling the intricacies of metabolic processes within live cells. Discover how these advanced tools not only provide valuable insights into cellular energetics but also offer a streamlined approach, saving you time and resources. Don't miss the opportunity to explore the forefront of live cell metabolic profiling with Agilent Seahorse, presented by experts in the field.



Download the Seahorse XF Instrument Selection Guide
Download the Agilent Seahorse XF Assay Kits & Reagents Overview
Download the new Media Selection Guide
Download the Seahorse Plastics Brochure


Agilent Technologies, Inc.


Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

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