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Logos Biosystems Inc.

LUNA-FL™ Dual Fluorescence Cell Counter

Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.

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Technology

With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

What the Luna-FL™ does best

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TWO COUNTERS IN ONE

The LUNA-FL™ can count in both brightfield and fluorescence mode, making it Logos' most versatile counter to date.

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COMPATIBLE WITH MOST CELLS

Advanced imaging software and GFP and RFP fluorescence optics sensitively detect fluorescence stained cells.

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GFP TRANSFECTION ANALYSIS

High resolution images are captured and analyzed to detect GFP-positive and -negative cells.

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MAXIMUM 30 SECONDS

Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed

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THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.

Supplier

Logos Biosystems Inc.

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Multimedia

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Experimental condition

HL-60 (50% viability)

HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

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Experimental condition

HL-60 (high density)

A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

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Human peripheral blood

Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.

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Experimental condition

Jurkat

Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

News & Events

News

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Logos Biosystems

LUNA™ Family Awarded Gold Seal of Quality

The LUNA family of automated cell counters from Logos Biosystems was awarded the SelectScience® Gold Seal of Quality at a special awards reception during the 2019 AACC annual scientific meeting and clinical lab expo in Anaheim, California, USA. SelectScience Seals of Quality are given to top-rated products that meet specific criteria based on the number and average rating of user reviews.

The LUNA family is the first set of cell counters to receive seals of quality for customer satisfaction and is now one of just seven products worldwide to have been recognized with a gold seal.

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